In vitro regeneration of banana
Shoot tip of BARI Banana-1 (Musa sp.) was cultured on MS medium supplemented with 5.0 mg/l BAP for shoot proliferation. Well developed shoots were used for rooting. Among the six different concentrations of IBA (0.1,0.2,0.3,0.4,0.5,0.6 mg/l) with half strength MS medium, a good number of healthy roots (7.86) were produced on half MS + 0.5 mg/l IBA. 95-100 percent plantlets were survived when they were transferred to small plastic pots after 15 - 20 days in vitro culture and 7 days hardening at room temperature.

b) Multiple shooting

d) Plantlet

In vitro regeneration of jackfruit
Shoot tip from matured tree of jackfruit (Artocarpus heterophyllus Lam) were cultured on MS medium supplemented with different concentrations of BAP. The highest multiple shoot was observed in 2.0 mg/l BAP. Rooting of in vitro regenerated shoot was made in half MS medium with different concentrations of IBA. Good rooting was obtained in 2.0 mg/l IBA.

b) Multiple shooting

In vitro regeneration of pineapple
The response of explant to various combinations of auxin and cytokinin in the MS medium were studied. A combination of 1.76 mg/l NAA, 1.93 mg/l IBA and 2.0 mg/l Kn was found optimum for establishment of cultures and subsequent shoot formation. The overall roots and plant growth were also found excellent in medium containing 0.2 mg/l NNA and 0.4 mg/l IBA.

Regeneration of teasle gourd (Momordica dioica Roxb) through tissue culture
Four types of explants of teasle gourd viz. node, shoot tip, leaf and the cotyledon were used in this study. The cotyledon showed best performance. The combination of 1.0 mg/l BAP and 0.1 mg/l/NAA were found most suitable in callus induction. The highest number of multiple and tallest shoots were obtained on MS medium supplemented with 1.0 mg/l BAP and 0.1 mg/l NAA. Half strength MS medium supplement with 0.3 mg/l IBA showed better performance for root induction.

In vitro culture of watermelon
In vitro grown shoot tips and nodal segments from physiologically mature seeds were cultured aseptically on MS medium supplemented with different concentrations of cytokinin (BAP and Kn) and auxin (IBA and NAA) for regeneration. Shoot tips were found better than nodal segments for shoot induction. The highest number of multiple shoots (15.4) and longest shoot (5.2 cm) were obtained on MS medium supplemented with 0.2 mg/l BAP. The highest number of roots/culture (7.5) and the longest root (1.9 cm) were produced in ½ MS medium supplemented with 0.4 mg/l IBA.

Tissue culture producing mature
plant of watermelon

In vitro regeneration of chrysanthemum
Shoot tips of Chrysanthemum morifolium ramat cv. 'super yellow' and 'light violet' was periodically subcultured on IAA, NAA, Kn, BAP and coconut water (CW) singly or in combinations. Maximum explants regeneration was observed in cv. super yellow and light violet from MS media supplemented with 0.8 mg/l Kn + 2.0 mg/l BAP + 0.5 mg/l IAA + 0.5 mg/l NAA + 10% CW and 0.8 mg/l Kn + 0.5 mg/l IAA + 10% CW combinations, respectively. Cultivar light violet gave highest number of multiple shoots per culture on MS medium supplemented with 5.0 mg/l BAP + 0.5 mg/l NAA. The maximum number of root was found in cv. super yellow and light violet from the half strength MS medium supplemented with 0.2 mg/l IBA and 0.2 mg/l IAA, respectively. The rooted shoots were acclimatized and successfully transferred to plastic pots. After hardening, they were transferred to the main field and the survival rate was observed 85%.

c) Rooting and Plantlet

In vitro regeneration of gladiolus
Flower stalk and cormel tip explants were tested for in vitro propagation of gladiolus. The nodal segments of flower stalk produced higher number of shoots when cultured on MS medium supplemented with 1.0 mg/l BAP. Shoot length was also highest at the same concentration. Regenerated shoots produced roots and induced large cormlets at the basal end when 2.0 mg/l IBA and 60 g/l sucrose were added to half MS medium. In vitro cormlets germinated in the field and produced flowers.

In vitro culture of orchids
Seed of Dendrobium hybrid orchid were cultured on MS medium supplemented with different concentrations and combinations of growth regulators. Highest percentage of seed germination was observed in 2.0 mg/l NAA. Highest shoot length, leaf number and root length were observed in 0.5 mg/l IAA + 1.0 mg/l IBA.

c)Multiple shooting

d) Rooting

In vitro propagation of rose
Node culture of hybrid tea (Peace, Virgo, Inhar Canex), climbers (Crimson Glory) and miniature (Sunburst red) roses were cultured on MS medium supplemented with different concentrations and combinations of BAP and Kn. Kinetin was found less effective than BAP for all cultivars. However, the best response of shoot multiplication was observed at Sunburst Red with 1.5 mg/l BAP. Crimson Glory, Inhar Canex and Peace showed intermediate response at 0.5 mg/l BAP with 0.2 mg/l Kn. Rooting occurred within 5 weeks in all cultivars. But the highest percentage of rooting was obtained in Peace (90%) at 0.5 mg/l IBA. The combination of IAA (0.5 mg/l) and IBA (0.5 mg/l) showed intermediate result (50%) for rooting in Crimson Glory and Sunburst red.

b) Rooting